Tollgene.org is an online repository for microarray, flow cytometry and secreted cytokines data from in vitro stimulated human whole blood.
Notes: Human blood can contain infectious agents. Blood needs to be manipulated in a biosafety cabinet, not on the bench, by properly trained personnel. Applying the aseptic technique will minimize the possibility of external contamination of cultures, materials and reagents. The footnote lists the vendors and catalog numbers for the consumables used in the experiments, but does not represent the endorsement of a particular source over other comparable products.
Note: It is recommended to create an Excel spreadsheet with all the stimuli, their stock concentrations, working concentrations and volumes, so calculations can be easily adjusted depending on the number of samples.
Note: If the reagents and consumables are not tested for endotoxin, 10 µg/mL of polymyxin B can be added to wells to prevent unintended TLR4 activation with endotoxin (LPS).
Note: After Tempus reagent is properly mixed in, the color of blood changes from deep red to very dark brown with a reddish hue.
Note: If the lid does not seal the plate tightly, place the plate in an additional leak-proof biohazard bag or container.
The heatmap represents hierarchical clustering of the reference module fingerprints, derived from microarray data of in vitro stimulated healthy adult blood using a method described here http://tinyurl.com/h8uxept. Module score is expressed on a transparency scale as a percentage of represented transcripts that are over- (red) or underexpressed (blue) more than two-fold. Data are normalized to the medium control for each donor. Fingerprints are averaged by stimulus. Median n (donor) per stimulus = 8.
*Click on a module to display its annotations and probe content.